Journal: Journal of biomolecular screening

Article Title: Discovery of Enhancers of the Secretion of Leukemia Inhibitory Factor for the Treatment of Multiple Sclerosis.

doi: 10.1177/1087057116638821

Figure Lengend Snippet: Figure 4. Correlation across assays of 200 selected compounds from HTS. (A) LIF production activation fold in U87-MG cells, AlphaScreen vs. ELISA (r2 = 0.753). (B) LIF production activation fold in AlphaScreen assay format, U87-MG vs. WM-115 cells (r2 = 0.347). Shape by chemical clustering: circles, cluster 3; squares, cluster 5; triangle, other cluster.

Article Snippet: Rat LIF ELISA kit was from Cusabio (Wuhan, China).

Techniques: Activation Assay, Amplified Luminescent Proximity Homogenous Assay, Enzyme-linked Immunosorbent Assay

Journal: Veterinary Research

Article Title: Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines

doi: 10.1186/s13567-017-0423-8

Figure Lengend Snippet: Specific binding of anti-chCD205 and internalization of antigen delivery complex. A The recombinant parts of chicken CD205 receptor used for monoclonal antibody preparation and the monoclonal antibody characterization. The CD205B and CD205F proteins were purified from inclusion bodies on DEAE Sepharose and separated on Tris-Tricine polyacrylamide gel stained by Coomassie blue (left). Monoclonal mouse anti-CD205 antibodies were analyzed using Western blots. Results of two representative detections of monoclonal anti-CD205 specific for CD205B or CD205F are shown. B CD205 expression on leukocytes. Splenocytes and peripheral leukocytes were stained with anti-chicken CD205 antibody and antibodies recognizing each of the indicated antigens. Histograms represent the CD205 fluorescence for cells gated for positive expression of the markers indicated at the left. The results of one representative of three experiments are shown. C Median fluorescence intensity (MFI) of CD205 stained cells and standard deviation. D Internalization of streptavidin-CD205-FITC complex by chicken primary splenocytes one (left) and two (right) hours of incubation in different temperatures. Dotted line plot non-labelled cells (biot—control IgG); Black line plot—FITC labelled cells incubated in 4 °C; Gray line plot—FITC labelled cells incubated in 40 °C.

Article Snippet: For each cytokine, the recombinant protein, capture antibody and detection antibody were obtained as complete kit (Kingfisher Biotech, USA; cat.nos.

Techniques: Binding Assay, Recombinant, Purification, Staining, Western Blot, Expressing, Fluorescence, Standard Deviation, Incubation, Control